Our Nature Methods paper is now online!!
In collaboration with Guido Sanguinetti’s group we developed new statistical algorithms for calling modified nucleotides in high-throughput RNA structure probing data. These new tools greatly increase the sensitivity of RNA structure probing methods, even at low coverage. The next challenge is to modify this method so that we can use it to determine which nucleotides undergo conformational changes under different conditions or during assembly of large macromolecular complexes, such as the ribosome. Can’t wait!!